Job ID 12382 Location Case Main Campus Full/Part Time Full-Time Regular/Temporary Regular Job Description POSITION OBJECTIVE Working under moderate supervision, the Research Assistant 1 will perform research work in medical and related technical areas. The research assistant will maintain specialized knockout and transgenic mice uniquely developed in the lab for studies in basic immunology. The research assistant will also perform basic immunology work to look at inflammatory molecules in order to discover underlying pathogenic mechanisms that cause chronic intestinal inflammation, such as that observed in patients with inflammatory bowel disease. This position will work with animals. ESSENTIAL FUNCTIONS Perform research work in medical and related technical areas involves use of lab skills. Maintain, organize, and perform minor husbandry of animal colonies. This includes but is not limited to providing care for animals, inspecting animals regularly, maintaining ID/records of all animals, and keeping datasheets up-to-date and circulated appropriately. Requires extensive and delicate handling of animals. (25%) Conduct analysis of samples. These include measurements of inflammatory molecules at the gene levels (by quantitative polymerase chain reaction experiments) and at the protein levels (by Western blot experiments, enzyme-linked immunosorbent assay; ELISA, or immunohistochemistry techniques). (20%) Maintain test equipment utilized in experiments. Aid in managing samples, including tissue and biological fluid (i.e., blood) collection, processing and accurately recording data associated with samples and research. (15%) Record results for a particular experiment or closely related series of experiments by using software, such as Graphpad Prism, or Excel. (15%) Maintain records of experiments. Keep detailed and organized records of experiments, protocols and procedures, and laboratory equipment utilized in experiments and the collected data. Organize shipment of samples from or to the laboratory. Maintain certification and servicing of laboratory equipment. (10%) Order general laboratory supplies and maintain inventory. (10%) NONESSENTIAL FUNCTIONS Prepare solutions, buffers, and media for use in experiments as needed. (4%) Perform other assigned duties. Interact with the administrative office or other departments for issues regarding safety, budget, purchase orders and department organization. (1%) CONTACTS Department: Frequent contact with supervisor to discuss research and maintain workflow. Frequent contact with lab colleagues to discuss research and maintain workflow. University: Occasional contact with other departments to share information. External: Occasional contact with vendors to exchange information. Students: Occasional Contact with student employees to exchange information. SUPERVISORY RESPONSIBILITY This position has no direct supervision of staff employees. QUALIFICATIONS Education/Experience: Bachelor's degree in science and 0 to 1 year of experience or Associates degree in an approved biotechnology program and 0 to 2 years of experience required. REQUIRED SKILLS Experience with proper animal handling and husbandry. Familiarity with safe handling of biohazardous materials (human blood and body fluids); and knowledge of safe handling practices for laboratory chemicals. Experience with basic laboratory equipment (centrifuge, light microscopes, balances, pH meter, incubators, etc.) and ability to learn new techniques and procedures as needed. Ability to closely follow established protocols or laboratory procedures and request clarification if necessary. Exceptional organizational skills in record-keeping with ability to organize, and accurately document data. Must have the ability to maintain meticulous, complete, and easily retrie